This recombinant 5hmC antibody reacts to 5-hydroxymethylcytosine in both single-stranded and double-stranded DNA. No cross reactivity with non-methylated cytosine and methylcytosine in DNA.
Formulation
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
BSA-conjugated 5-hydroxymethylcytosine was used as the immunogen for this recombinant 5hmC antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
The stated application concentrations are suggested starting points. Titration of the recombinant 5hmC antibody may be required due to differences in protocols and secondary/substrate sensitivity. 1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Uniprot
N/A
Format
Purified
Purity
Protein A purified from animal origin-free supernatant
Storage
Store the recombinant 5hmC antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).