This recombinant phospho-EGFR antibody reacts to human EGF Receptor only when phosphorylated at Tyr1173. There is no cross-reactivity to EGFR without phosphorylation at Tyr1173.
Formulation
Antibody in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
A phospho-peptide corresponding to human phospho- EGFR (Tyr1173) was used as the immunogen for this recombinant phospho-EGFR antibody.
Isotype
Rabbit IgG
Species Reactivity
Human
Note
The stated application concentrations are suggested starting points. Titration of the recombinant phospho-EGFR antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Uniprot
P00533
Format
Purified
Purity
Protein A purified from animal origin-free supernatant
Storage
Store the recombinant phospho-EGFR antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).