This recombinant Phosphothreonine antibody reacts with threonine-phosphorylated proteins. No cross reactivity with nonphosphorylated threonine, phosphoserine, and phosphotyrosine. It shows slight cross-reactivity with a few phospho-serine-containing peptides.
Formulation
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
Mixture of phosphothreonine-BSA conjugate and a phosphothreonine containing peptide was used as the immunogen for this recombinant Phosphothreonine antibody.
Isotype
Rabbit IgG
Species Reactivity
All Species
Note
The stated application concentrations are suggested starting points. Titration of the recombinant Phosphothreonine antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Format
Purified
Purity
Protein A purified from animal origin-free supernatant
Storage
Store the recombinant Phosphothreonine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Applications
WB, IP, ICC, IHC, ChIP
Description
Western blot: 1:500 -1:2000 dilution,Immunoprecipitation: 1:100-1:500 dilution,Chromatin IP (ChIP): 1:100-1:500 dilution,Immunocytochemistry: 1:100-1:500 dilution,Immunohistochemistry: 1:100-1:500 dilution (1)