This recombinant Phosphotyrosine antibody reacts to tyrosine-phosphorylated proteins. No cross reactivity with nonphosphorylated tyrosine, phosphoserine, and phosphorthreonine seen.
Formulation
1 mg/ml in PBS with 50% glycerol, 1% BSA and 0.09% sodium azide
Host
Rabbit
Immunogen Region
Phosphotyrosine-BSA conjugate was used as the immunogen for this recombinant Phosphotyrosine antibody.
Isotype
Rabbit IgG
Species Reactivity
All Species
Note
The stated application concentrations are suggested starting points. Titration of the recombinant Phosphotyrosine antibody may be required due to differences in protocols and secondary/substrate sensitivity.1. A pH6 Citrate buffer or pH9 Tris/EDTA buffer HIER step is recommended for testing of FFPE tissue sections.
Format
Purified
Purity
Protein A purified from animal origin-free supernatant
Storage
Store the recombinant Phosphotyrosine antibody at -20oC (with glycerol) or aliquot and store at -20oC (without glycerol).
Applications
WB, IP, ICC, IHC, ChIP
Description
Western blot: 1:1000 -1:5000,Immunoprecipitation: 1:200-1:1000,Chromatin IP (ChIP): 1:200-1:1000,Immunocytochemistry: 1:200-1:500,Immunohistochemistry: 1:200-1:500 (1)