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ELK Biotechnology
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ELK Biotechnology
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| Mfr.No. | ELK9080MS | |||||||||||||||||||||||||||
| Sensitivity | 9.8 pg/mL | |||||||||||||||||||||||||||
| Detection Range | 31.25-2000 pg/mL | |||||||||||||||||||||||||||
| Reactivity | Human | |||||||||||||||||||||||||||
| Assay Duration | 3.5h | |||||||||||||||||||||||||||
| Assay Type | Sandwich | |||||||||||||||||||||||||||
| Alternative Names | High Sensitivity Cardiac Troponin I | |||||||||||||||||||||||||||
| Kit Components |
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| Linearity |
The linearity of the kit was assayed by testing samples spiked with appropriate concentration of hs-cTnI and their serial dilutions. The results were demonstrated by the percentage of calculated concentration to the expected.
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| Standard Curve |
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| Sample Type | Serum, Plasma, Tissue homogenates, Cell lysates, Cell culture supernatants, Other biological fluids | |||||||||||||||||||||||||||
| Limit Of Detection | 2000 pg/mL | |||||||||||||||||||||||||||
| Precision |
Intra-assay Precision (Precision within an assay)?CV%<8% Three samples of known concentration were tested twenty times on one plate to assess intra-assay precision. Inter-assay Precision (Precision between assays)?CV%<10% Three samples of known concentration were tested in forty separate assays to assess inter-assay precision. |
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| Usage Notes | The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human hs-cTnI. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human hs-cTnI. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human hs-cTnI, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human hs-cTnI in the samples is then determined by comparing the OD of the samples to the standard curve. | |||||||||||||||||||||||||||
| Protocol | Manual |
| SKU | Supplier No. | Size | Your price | Quantity | |
|---|---|---|---|---|---|
| BHE15210591 | ELK9080MS | 48T | $535.60 |
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| BHE15210591 | ELK9080MS | 96T | $764.40 |
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| BHE15210591 | ELK9080MS | 96T*5 | $3248.70 |
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