FLAG-Tag, or FLAG octapeptide, or FLAG epitope, is a polypeptide protein tag that can be added to a protein using recombinant DNA technology, having the sequence motif DYKDDDDK. It represents an early example of a nanotechnology device, in that it can be attached to a protein like a molecular handle, then used to manipulate the protein, and subsequently detached. It has been used for studying proteins in living cells and for protein purification by affinity chromatography.
Formulation
0.5mg/ml in PBS with 0.02% sodium azide and 50% glycerol
Host
Mouse
Immunogen Region
Amino acids DYKDDDDK were used as the immunogen for the FLAG Tag antibody.
Isotype
IgG2b
Predicted Reactivity
DYKDDDDK
Reactivity
DYKDDDDK
Recombinant
No
Antigen
FLAG Tag
Clone No
1E6-
Format
Purified
Purification
Protein G affinity
Storage
After reconstitution, the FLAG Tag antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.