The paxillin gene can be alternatively spliced to include 1 of 2 alternative exons, generating beta and gamma isoforms. Paxillin is a 68-kDa focal adhesion protein that is phosphorylated on tyrosine residues in fibroblasts in response to transformation by v-src, treatment with platelet-derived growth factor, or cross-linking of integrins. The 68-kD protein(paxillin) is a cytoskeletal component that localizes to the focal adhesions at the ends of actin stress fibers in chicken embryo fibroblasts. It is also present in the focal adhesions of Madin-Darby bovine kidney(MDBK) epithelial cells but is absent, like talin, from the cell-cell adherens junctions of these cells.
Formulation
0.5mg/ml with 1% BSA and 0.01% sodium azide if reconstituted with 0.2ml sterile 1XPBS
Host
Mouse
Immunogen Region
C-terminal partial recombinant chicken paxillin (amino acids 305-559) was used as the immunogen for this Paxillin antibody.
Isotype
IgG1
Predicted Reactivity
Human, mouse, rat, chicken
Reactivity
Human, Mouse, Rat, Chicken
Recombinant
No
Antigen
Paxillin
Gene Id
5829
Clone No
PXC-10
Format
Ascites
Purification
Ascites
Storage
After reconstitution, the Paxillin antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
ICC, WB
Dilution
Western blot: 1-2ug/ml,Immunocytochemistry: Suitable