Prominin-1, also known as CD133, is a glycoprotein that in humans is encoded by the PROM1 gene. It is mapped to 4p15.32. Prominin-1 is a member of pentaspan transmembrane glycoproteins (5-transmembrane, 5-TM), which specifically localize to cellular protrusions. This gene encodes a pentaspan transmembrane glycoprotein. The protein localizes to membrane protrusions and is often expressed on adult stem cells, where it is thought to function in maintaining stem cell properties by suppressing differentiation. It has been proposed to act as an organizer of cell membrane topology. Prominin-1 was expressed not only on metastatic colon cancer cells, but also on differentiated colonic epithelium in both adult mice and humans.
Formulation
0.5mg/ml if reconstituted with 0.2ml sterile DI water
Host
Mouse
Immunogen Region
A human recombinant partial protein (amino acids P531-H865) was used as the immunogen for the PROM1 antibody.
Isotype
IgG2a
Predicted Reactivity
Human, Mouse
Reactivity
Human, Mouse
Recombinant
No
Antigen
CD133
Uniprot
O43490
Clone No
8B6
Format
Antigen affinity purified
Purification
Affinity purified
Storage
After reconstitution, the PROM1 antibody can be stored for up to one month at 4°C. For long-term, aliquot and store at -20°C. Avoid repeated freezing and thawing.
Applications
WB, FACS
Dilution
Western blot: 1-2ug/ml,Flow cytometry: 1-3ug/million cells