CLS Cell Line

Human Ductal carcinoma cell line CCD-1095Sk

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2 m
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Tissue : Skin

Human Endometrial adenocarcinom cell line KLE

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 5% FBS, w: 3.1
• Antigen Expression : Blood Type O, R
• Passaging Solution : Accutase
• Biosafety level : 1

Human Endometrial adenocarcinoma cell line HEC-1-A

• Handling of Cryopreserved Cultures : The cells come
• Receptors Expressed : Receptor expres
• Protein Expression : Oncogenes: C-Fo
• Medium Supplements : 10% FBS, w: 3.0
• Antigen Expression : Blood Type B, R

Human Endometrial adenosquamous carcinoma cell line RL95-2

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 5% FBS, w: 3.1
• Passaging Solution : Accutase
• Biosafety level : 1
• Citation : RL95-2 (CLS cat

Human Epidermoid Carcinoma cell line ME-180

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 3.0
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Human Erythroleukemia cell line HEL

• Subculturing : Collect suspens
• Medium Supplements : 10% FBS, w: 2.1
• Passaging Solution : Accutase
• Handling of Cryopreserved Cultures : The cells come
• Handling of Proliferating Cultures : One or two cell

Human Esophageal squamous cell carcinoma cell line KYSE-30

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS
• Passaging Solution : Accutase
• Biosafety level : 1
• Citation : KYSE-30 (CLS ca

Human Esophageal squamous cell carcinoma cell line KYSE-150

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS
• Passaging Solution : Accutase
• Biosafety level : 1
• Citation : KYSE-150 (CLS c

Human Esophageal squamous cell carcinoma cell line KYSE-410

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2.1
• Passaging Solution : Accutase
• Biosafety level : 1
• Citation : KYSE-410 (CLS c

Human Esophageal squamous cell carcinoma cell line TE-1

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2.1
• Passaging Solution : Accutase
• Biosafety level : 1
• Citation : TE-1 (CLS catal

Human Gastric adenocarcinoma cell line MKN-45

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 20% heat-inacti
• Passaging Solution : Accutase
• Biosafety level : 1
• Citation : MKN-45 (CLS cat

Human Gastric tubular adenocarcinoma cell line N87

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2.1
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Human Pancreas Adenocarcinoma cell line L428

• Handling of Proliferating Cultures : One or two cell
• Medium Supplements : 10% FBS, w: 2.1
• Passaging Solution : The utilization
• Handling of Cryopreserved Cultures : The cells come
• Subculturing : Maintain cultur

Mouse cell line L Wnt-3A

• Handling of Cryopreserved Cultures : The cells come
• Protein Expression : Wnt-3A
• Medium Supplements : 10% FBS, w: 4.5
• Passaging Solution : Accutase
• Biosafety level : 1

Mouse cell line LM/TK(LMTK-)

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 4.5
• Antigen Expression : H-2k
• Passaging Solution : Accutase
• Biosafety level : 1

Mouse cell line TM4

• Handling of Cryopreserved Cultures : The cells come
• Receptors Expressed : Follicle stimul
• Protein Expression : Retinol Binding
• Medium Supplements : 2.5% FBS, 5% ho
• Antigen Expression : H-Y antigen, Mu

Mouse, transgenic cell line 15P-1

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 4.5
• Passaging Solution : Accutase
• Biosafety level : 1
• Citation : 15P-1 (CLS cata

Pig cell line ST

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2 m
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Human cell line EA.hy926

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 4.5
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Animal cells cell line MR1

• Passaging Solution : The utilization
• Protein Expression : immunoglobulin,
• Medium Supplements : 10% FBS, w: 2.1
• Handling of Cryopreserved Cultures : The cells come
• Handling of Proliferating Cultures : One or two cell

Mouse cell line MEL

• Passaging Solution : The utilization
• Handling of Cryopreserved Cultures : The cells come
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Resuspend cell
• Split Ratio : 1:3 to 1:5

Mouse cell line P3/NSI/1-Ag4-1

Human Diffuse large B-cell lymphoma cell line DB

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2.1
• Passaging Solution : The utilization
• Biosafety level : 1
• Citation : DB (CLS catalog

Human Diffuse large B-cell lymphoma cell line SU-DHL-4

• Handling of Cryopreserved Cultures : The cells come
• Protein Expression : IgG+, Kappa+, I
• Medium Supplements : 10% FBS, w: 2.1
• Passaging Solution : The utilization
• Biosafety level : 1

Human Hodgkin lymphoma cell line L-591

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2.1
• Passaging Solution : The utilization
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Maintain cultur

Human Mantle cell lymphoma cell line JeKo-1

• Handling of Cryopreserved Cultures : The cells come
• Protein Expression : Cd3-, Cd5?, Cd1
• Medium Supplements : 20% heat-inacti
• Passaging Solution : The utilization
• Handling of Proliferating Cultures : One or two cell

Human Sezary syndrome (aggressive form of cutaneous T-cell lymphoma) cell line H9

• Handling of Proliferating Cultures : One or two cell
• Medium Supplements : 10% FBS, w: 2.1
• Isoenzymes : CD4+
• Virus Susceptibility : HIV-1 (HTLV-III
• Protein Expression : Interleukin 2 (

Human T-cell acute lymphoblastic leukemia cell line Jurkat

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, 0.05 m
• Passaging Solution : The utilization
• Biosafety level : 1
• Citation : Jurkat (CLS cat

Human cell line Farage

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2.1
• Biosafety level : 2
• Citation : Farage (CLS cat
• Metastatic Site : Lymph node

Human cell line MRC-5

• Handling of Cryopreserved Cultures : The cells come
• Virus Susceptibility : Not susceptible
• Medium Supplements : 10% FBS, w: 2 m
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell

Human cell line HFL1

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2.0
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Human cell line BEAS-2B

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Tissue : Lung

Rat cell line H9c2(2-1)

• Handling of Cryopreserved Cultures : The cells come
• Protein Expression : Myokinase, Crea
• Receptors Expressed : Acetylcholine,
• Medium Supplements : 10% FBS, w: 4.5
• Passaging Solution : Accutase

Mouse cell line L-138

• Handling of Proliferating Cultures : One or two cell
• Medium Supplements : 10% FBS, w: 2.1
• Passaging Solution : The utilization
• Handling of Cryopreserved Cultures : The cells come
• Subculturing : Start cultures

Cat cell line F81

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2 m
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Cercopithecus aethiops cell line COS-1

• Handling of Cryopreserved Cultures : The cells come
• Protein Expression : T Antigen, This
• Medium Supplements : 10% FBS, w: 4.5
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell

Chlorocebus pygerythrus cell line BS-C-1

• Handling of Cryopreserved Cultures : The cells come
• Protein Expression : Keratin
• Medium Supplements : 10% FBS, w: 2 m
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell

Human cell line HEK293T

• Handling of Cryopreserved Cultures : The cells come
• Protein Expression : CEA negative, p
• Receptors Expressed : Vitronectin
• Medium Supplements : 10% FBS, w: 2 m
• Passaging Solution : Accutase

Rat cell line NRK-52E

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 4.5
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Rat cell line HBZY-1

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 4.5
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Rhesus macaque cell line LLC-MK2

• Handling of Cryopreserved Cultures : The cells come
• Protein Expression : Plasminogen act
• Medium Supplements : 1% horse serum,
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell

Human cell line HK-2

• Handling of Cryopreserved Cultures : The cells come
• Receptors Expressed : Epidermal growt
• Protein Expression : Alkaline Phosph
• Medium Supplements : 10% FBS, 10 ?g/
• Passaging Solution : Accutase

Mouse cell line SV40 MES 13

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 4.5
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Human cell line LX-2

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 4.5
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Mouse cell line AML12

• Medium Supplements : 5% FBS, w: 3.1
• Passaging Solution : Accutase
• Handling of Cryopreserved Cultures : The cells come
• Handling of Proliferating Cultures : One or two cell
• Growth Properties : Adherent

Rat cell line BRL

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2 m
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

American Mink cell line Mv.1.Lu

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 2 m
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Hamster cell line V79

• Handling of Cryopreserved Cultures : The cells come
• Medium Supplements : 10% FBS, w: 4.5
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell
• Subculturing : Remove medium a

Mouse cell line MH-S

• Handling of Cryopreserved Cultures : The cells come
• Protein Expression : interleukin 1 (
• Medium Supplements : 10% FBS, w: 2.1
• Passaging Solution : Accutase
• Handling of Proliferating Cultures : One or two cell

Rat cell line NR8383

• Subculturing : Collect suspens
• Protein Expression : Transforming Gr
• Receptors Expressed : Fc
• Medium Supplements : 15% FBS, w: 2.0
• Passaging Solution : Accutase